35:1,(2.07um Amino bead -Thiol-DNA-Biotin-Streptavidin bead 3.05um ) No3 Rise, pleatue, (bead came out of trap) New distance recognition. Distance offset (perp) = 9275 Time (ms) Elongation (nm) Force Dual (pN) Relative (nm) 1197.00 3944.70 0.26 -2.73 -0.31 0.05 4.63 4.45 1300.00 3956.94 -0.51 4.51 -1.17 1.68 4.63 4.46 1312.00 3964.15 -0.68 6.72 -0.23 0.91 4.63 4.46 1334.00 3966.29 -0.43 3.87 -0.87 1.30 4.63 4.45 1352.00 3967.09 -0.01 -0.34 -0.95 0.96 4.62 4.45 1367.00 3972.66 -0.08 0.23 -1.12 1.20 4.62 4.46 1380.00 3979.85 -0.28 2.42 -0.88 1.16 4.63 4.46 1394.00 3986.89 -0.49 4.46 -0.86 1.35 4.63 4.46 1407.00 3997.42 -1.03 9.99 -0.57 1.60 4.63 4.46 1421.00 3994.48 -0.26 2.06 -1.06 1.31 4.63 4.46 1438.00 4000.32 -0.33 2.89 -0.87 1.20 4.63 4.46 1448.00 4007.11 -0.49 4.68 -0.38 0.87 4.63 4.46 1458.00 4008.35 -0.10 0.92 -0.11 0.21 4.64 4.46 1471.00 4008.20 0.41 -4.23 -0.42 0.02 4.63 4.46 1482.00 4018.28 -0.09 0.86 -0.04 0.13 4.64 4.46 1498.00 4022.02 0.03 -0.41 -0.16 0.13 4.64 4.46 1511.00 4029.55 -0.24 2.12 -0.50 0.73 4.63 4.46 1524.00 4033.91 -0.16 1.48 -0.18 0.33 4.64 4.46 1536.00 4040.43 -0.35 3.00 -1.04 1.39 4.63 4.46 1547.00 4044.23 -0.22 1.81 -0.85 1.08 4.63 4.46 1558.00 4048.97 -0.16 1.54 -0.11 0.27 4.63 4.46 1569.00 4051.43 0.09 -1.00 -0.24 0.15 4.63 4.46 1580.00 4052.84 0.41 -4.59 -1.16 0.75 4.63 4.46 1591.00 4059.55 0.23 -2.88 -1.27 1.04 4.62 4.46 1601.00 4066.48 0.05 -0.95 -0.86 0.80 4.63 4.46 1612.00 4075.04 -0.25 2.61 0.18 0.08 4.64 4.46 1625.00 4078.91 -0.15 1.48 -0.03 0.18 4.64 4.46 1636.00 4079.08 0.29 -3.35 -0.99 0.70 4.63 4.46 1646.00 4085.66 0.12 -1.77 -1.24 1.13 4.63 4.47 1659.00 4091.13 0.07 -1.30 -1.25 1.18 4.62 4.46 1672.00 4099.89 -0.30 2.46 -1.17 1.47 4.62 4.46 1683.00 4106.31 -0.40 3.88 -0.18 0.57 4.63 4.47 1694.00 4108.24 -0.09 0.81 -0.30 0.39 4.63 4.46 1705.00 4113.44 -0.14 1.01 -0.90 1.05 4.63 4.46 1716.00 4120.39 -0.35 2.97 -1.09 1.44 4.63 4.46 1726.00 4128.23 -0.61 5.80 -0.55 1.16 4.63 4.47 1737.00 4129.60 -0.23 2.17 -0.23 0.45 4.63 4.46 1750.00 4139.88 -0.74 7.45 0.17 0.57 4.64 4.47 1761.00 4133.57 0.35 -3.85 -0.80 0.45 4.63 4.46 1772.00 4146.23 -0.43 3.80 -1.11 1.55 4.63 4.46 1783.00 4157.41 -1.03 9.98 -0.56 1.59 4.63 4.47 1794.00 4159.55 -0.74 7.12 -0.59 1.34 4.63 4.46 1805.00 4159.59 -0.23 2.16 -0.27 0.50 4.64 4.46 1816.00 4163.15 -0.11 0.72 -0.80 0.91 4.63 4.46 1827.00 4174.61 -0.77 7.18 -0.96 1.73 4.63 4.46 1838.00 4177.20 -0.52 4.78 -0.90 1.42 4.63 4.46 1848.00 4177.26 -0.00 -0.17 -0.43 0.43 4.63 4.46 1859.00 4179.19 0.30 -3.24 -0.66 0.36 4.63 4.46 1872.00 4188.74 -0.15 1.31 -0.48 0.63 4.64 4.46 1885.00 4196.74 -0.45 4.32 -0.41 0.87 4.63 4.46 1896.00 4200.11 -0.33 2.69 -1.20 1.52 4.62 4.46 1907.00 4205.91 -0.41 3.48 -1.21 1.62 4.62 4.46 1919.00 4210.07 -0.28 2.64 -0.39 0.67 4.63 4.45 1929.00 4214.00 -0.16 1.57 -0.07 0.23 4.64 4.46 1943.00 4216.11 0.12 -1.32 -0.23 0.10 4.64 4.46 1956.00 4221.52 0.08 -0.90 -0.25 0.17 4.64 4.46 1969.00 4232.82 -0.54 5.39 0.04 0.50 4.64 4.46 1980.00 4229.51 0.23 -2.92 -1.25 1.01 4.63 4.46 1991.00 4245.23 -0.82 7.80 -0.71 1.53 4.63 4.46 2002.00 4246.32 -0.40 3.90 -0.30 0.70 4.63 4.46 2013.00 4253.01 -0.58 5.58 -0.48 1.06 4.63 4.46 2025.00 4246.51 0.54 -5.92 -1.26 0.72 4.63 4.46 2036.00 4259.01 -0.18 1.58 -0.48 0.66 4.63 4.45 2053.00 4260.67 0.15 -1.75 -0.62 0.48 4.63 4.46 2063.00 4267.49 -0.05 0.06 -0.98 1.03 4.63 4.46 2074.00 4275.25 -0.33 2.82 -1.08 1.42 4.62 4.46 2087.00 4280.31 -0.34 2.88 -1.07 1.41 4.62 4.46 2098.00 4282.83 -0.06 0.40 -0.38 0.44 4.63 4.46 2109.00 4284.66 0.26 -2.77 -0.37 0.11 4.63 4.46 2120.00 4288.87 0.34 -3.56 -0.48 0.14 4.63 4.46 2133.00 4299.03 -0.19 1.60 -0.68 0.87 4.63 4.46 2146.00 4297.10 0.48 -5.33 -1.09 0.61 4.63 4.46 2158.00 4306.49 0.04 -0.94 -1.16 1.12 4.62 4.46 2169.00 4313.59 -0.15 1.16 -0.70 0.85 4.63 4.46 2180.00 4317.46 -0.02 0.03 -0.37 0.39 4.63 4.46 2191.00 4320.91 0.13 -1.52 -0.41 0.27 4.63 4.46 2202.00 4326.17 0.07 -1.26 -1.16 1.09 4.63 4.46 2213.00 4333.56 -0.16 1.14 -0.85 1.00 4.63 4.47 2223.00 4333.87 0.34 -3.56 -0.45 0.11 4.63 4.47 2234.00 4344.38 -0.20 1.95 -0.03 0.22 4.64 4.47 2248.00 4345.14 0.22 -2.29 -0.14 -0.09 4.64 4.47 2261.00 4350.79 0.16 -1.64 -0.13 -0.03 4.64 4.46 2274.00 4353.09 0.42 -4.34 -0.32 -0.10 4.64 4.46 2286.00 4360.33 0.17 -2.10 -0.91 0.74 4.63 4.46 2297.00 4369.39 -0.25 1.96 -1.19 1.45 4.63 4.46 2308.00 4372.56 -0.05 0.13 -0.85 0.91 4.63 4.46 2319.00 4379.72 -0.23 2.29 -0.06 0.29 4.64 4.46 2330.00 4379.65 0.26 -2.78 -0.37 0.11 4.64 4.46 2341.00 4382.91 0.41 -4.51 -0.95 0.54 4.63 4.46 2352.00 4391.48 0.04 -0.94 -1.17 1.13 4.62 4.46 2363.00 4394.94 0.21 -2.49 -0.79 0.57 4.63 4.46 2375.00 4399.76 0.26 -2.67 -0.24 -0.02 4.64 4.46 2386.00 4402.41 0.50 -5.01 -0.03 -0.47 4.64 4.46 2396.00 4404.92 0.68 -7.51 -1.48 0.79 4.63 4.46 2407.00 4412.82 0.44 -4.61 -0.54 0.10 4.63 4.46 2424.00 4411.74 1.02 -10.69 -1.05 0.03 4.63 4.46 2435.00 4424.84 0.21 -2.59 -1.08 0.88 4.62 4.46 2446.00 4428.35 0.36 -4.07 -1.08 0.73 4.63 4.46 2462.00 4431.48 0.54 -5.95 -1.28 0.75 4.62 4.46 2474.00 4437.64 0.42 -4.79 -1.20 0.77 4.63 4.46 2488.00 4446.33 0.07 -1.09 -0.75 0.68 4.63 4.46 2502.00 4451.59 0.05 -0.83 -0.82 0.78 4.63 4.46 2512.00 4451.77 0.56 -5.66 -0.14 -0.42 4.64 4.46 2523.00 4453.54 0.86 -8.89 -0.62 -0.25 4.63 4.46 2537.00 4458.96 0.81 -8.47 -0.85 0.04 4.63 4.46 2550.00 4464.29 0.80 -8.14 -0.35 -0.45 4.63 4.46 2564.00 4471.10 0.63 -6.33 -0.21 -0.41 4.64 4.46 2578.00 4473.77 0.85 -8.66 -0.52 -0.33 4.63 4.46 2589.00 4481.02 0.59 -6.41 -1.09 0.49 4.62 4.46 2601.00 4486.70 0.52 -5.72 -1.17 0.65 4.62 4.46 2614.00 4492.20 0.46 -5.23 -1.42 0.97 4.62 4.46 2628.00 4496.47 0.55 -5.96 -0.97 0.42 4.62 4.47 2639.00 4497.20 0.99 -10.23 -0.87 -0.11 4.63 4.46 2650.00 4500.02 1.22 -12.41 -0.52 -0.70 4.63 4.46 2661.00 4506.37 1.06 -11.06 -1.05 -0.01 4.63 4.46 2672.00 4515.68 0.67 -6.75 -0.15 -0.53 4.64 4.46 2687.00 4516.93 1.00 -10.50 -1.11 0.11 4.63 4.46 2698.00 4518.72 1.30 -13.71 -1.59 0.29 4.62 4.46 2712.00 4526.75 1.01 -10.68 -1.20 0.19 4.62 4.46 2723.00 4537.35 0.49 -5.08 -0.32 -0.17 4.63 4.46 2734.00 4540.30 0.69 -7.13 -0.48 -0.22 4.63 4.46 2745.00 4538.98 1.30 -13.45 -1.03 -0.27 4.63 4.46 2756.00 4545.57 1.17 -11.86 -0.42 -0.75 4.63 4.46 2771.00 4553.97 0.80 -8.45 -1.15 0.35 4.63 4.46 2782.00 4556.68 1.02 -10.75 -1.30 0.29 4.63 4.46 2796.00 4562.27 0.95 -10.15 -1.52 0.57 4.62 4.46 2807.00 4566.02 1.08 -11.41 -1.37 0.29 4.62 4.46 2818.00 4568.94 1.32 -13.48 -0.77 -0.54 4.63 4.46 2829.00 4572.75 1.44 -14.68 -0.79 -0.64 4.63 4.46 2840.00 4576.38 1.58 -16.05 -0.75 -0.82 4.63 4.46 2855.00 4583.53 1.35 -13.90 -1.06 -0.29 4.63 4.46 2866.00 4588.71 1.31 -13.72 -1.42 0.11 4.63 4.46 2879.00 4591.28 1.55 -16.15 -1.45 -0.10 4.62 4.46 2890.00 4596.46 1.55 -15.97 -1.05 -0.50 4.62 4.46 2901.00 4602.63 1.45 -14.80 -0.73 -0.72 4.63 4.46 2912.00 4604.06 1.78 -18.37 -1.24 -0.55 4.62 4.46 2923.00 4608.71 1.83 -18.72 -1.12 -0.71 4.63 4.46 2938.00 4615.64 1.61 -16.79 -1.51 -0.11 4.63 4.46 2949.00 4620.31 1.66 -17.12 -1.27 -0.39 4.63 4.46 2966.00 4623.76 1.82 -18.67 -1.13 -0.69 4.63 4.46 2977.00 4631.25 1.54 -16.18 -1.79 0.25 4.62 4.46 2988.00 4635.00 1.66 -17.43 -1.90 0.25 4.62 4.46 2999.00 4640.50 1.66 -16.93 -0.82 -0.84 4.63 4.46 3010.00 4641.26 2.08 -21.17 -1.02 -1.06 4.63 4.46 3020.00 4643.94 2.27 -23.49 -1.86 -0.41 4.62 4.46 3031.00 4652.66 1.94 -19.77 -1.04 -0.90 4.63 4.46 3048.00 4655.21 2.15 -22.22 -1.63 -0.52 4.62 4.46 3059.00 4660.24 2.13 -22.19 -1.89 -0.25 4.62 4.46 3070.00 4668.47 1.83 -18.96 -1.43 -0.40 4.62 4.46 3081.00 4666.74 2.53 -25.69 -1.02 -1.51 4.63 4.46 3092.00 4670.12 2.65 -27.30 -1.77 -0.88 4.62 4.46 3107.00 4674.59 2.74 -27.84 -1.08 -1.66 4.62 4.46 3118.00 4681.41 2.54 -26.02 -1.42 -1.13 4.62 4.46 3136.00 4684.54 2.73 -27.89 -1.42 -1.31 4.62 4.46 3146.00 4695.68 2.11 -21.75 -1.42 -0.69 4.62 4.46 3157.00 4698.39 2.32 -24.04 -1.86 -0.46 4.62 4.46 3171.00 4703.75 2.28 -23.67 -1.90 -0.38 4.62 4.46 3182.00 4710.75 2.10 -21.68 -1.56 -0.54 4.62 4.46 3193.00 4708.82 2.78 -28.61 -1.89 -0.89 4.62 4.46 3204.00 4705.72 3.60 -36.71 -1.72 -1.88 4.62 4.46 3215.00 4715.84 3.09 -31.58 -1.60 -1.49 4.62 4.46 3226.00 4720.58 3.09 -31.85 -2.20 -0.89 4.61 4.46 3237.00 4724.31 3.20 -33.12 -2.46 -0.74 4.61 4.46 3248.00 4725.26 3.65 -37.17 -1.66 -1.99 4.62 4.46 3258.00 4729.53 3.70 -37.90 -2.03 -1.67 4.62 4.46 3272.00 4731.63 3.98 -40.80 -2.25 -1.74 4.61 4.46 3286.00 4739.51 3.70 -37.92 -2.15 -1.55 4.61 4.46 3303.00 4739.15 4.25 -43.28 -1.99 -2.26 4.61 4.46 3315.00 4752.59 3.38 -34.84 -2.36 -1.01 4.61 4.46 3332.00 4754.28 3.72 -38.15 -2.14 -1.59 4.61 4.47 3345.00 4755.70 4.09 -41.73 -1.98 -2.11 4.61 4.47 3359.00 4756.58 4.48 -45.85 -2.48 -2.00 4.61 4.46 3373.00 4764.84 4.12 -42.59 -2.97 -1.15 4.60 4.46 3389.00 4769.87 4.16 -42.56 -2.29 -1.86 4.61 4.46 3400.00 4767.09 4.93 -50.34 -2.45 -2.48 4.60 4.46 3414.00 4775.04 4.60 -47.39 -3.02 -1.58 4.60 4.46 3429.00 4779.72 4.65 -47.71 -2.66 -1.99 4.60 4.46 3443.00 4782.10 4.93 -50.33 -2.30 -2.64 4.60 4.46 3457.00 4785.22 5.09 -52.21 -2.81 -2.28 4.60 4.45 3469.00 4789.07 5.25 -53.35 -2.05 -3.20 4.60 4.46 3480.00 4791.64 5.45 -55.79 -2.78 -2.67 4.60 4.46 3498.00 4794.34 5.70 -58.09 -2.54 -3.15 4.60 4.46 3511.00 4803.86 5.21 -53.56 -3.20 -2.01 4.60 4.46 3528.00 4807.08 5.38 -55.35 -3.27 -2.11 4.60 4.46 3544.00 4808.53 5.73 -58.90 -3.31 -2.42 4.59 4.46 3555.00 4807.83 6.30 -64.60 -3.42 -2.88 4.59 4.46 3566.00 4816.92 5.90 -60.51 -3.23 -2.67 4.59 4.46 3583.00 4812.95 6.81 -69.48 -3.03 -3.77 4.59 4.46 3599.00 4819.21 6.63 -68.21 -3.87 -2.76 4.59 4.46 3613.00 4822.29 6.83 -70.14 -3.82 -3.01 4.59 4.46 3627.00 4828.16 6.76 -69.26 -3.49 -3.26 4.59 4.46 3637.00 4824.28 7.66 -78.15 -3.35 -4.31 4.59 4.46 3648.00 4836.37 6.93 -71.05 -3.54 -3.39 4.59 4.45 3666.00 4836.86 7.38 -75.57 -3.58 -3.80 4.59 4.46 3681.00 4841.40 7.40 -76.03 -3.97 -3.43 4.58 4.45 3695.00 4845.21 7.52 -77.22 -4.00 -3.52 4.58 4.45 3709.00 4848.35 7.70 -79.08 -4.03 -3.68 4.58 4.45 3721.00 4845.62 8.49 -86.81 -3.86 -4.63 4.58 4.45 3732.00 4851.00 8.41 -86.42 -4.49 -3.92 4.58 4.45 3750.00 4856.86 8.34 -85.57 -4.29 -4.05 4.58 4.45 3761.00 4850.22 9.52 -97.21 -3.90 -5.62 4.58 4.45 3773.00 4860.27 8.98 -92.16 -4.47 -4.52 4.58 4.45 3791.00 4862.82 9.22 -94.61 -4.69 -4.53 4.57 4.45 3806.00 4865.95 9.40 -96.48 -4.75 -4.65 4.57 4.45 3820.00 4868.91 9.61 -98.52 -4.64 -4.97 4.57 4.45 3834.00 4870.05 9.97 -102.38 -5.00 -4.98 4.57 4.45 3846.00 4869.30 10.57 -108.13 -4.64 -5.93 4.57 4.45 3857.00 4870.34 10.94 -112.08 -5.01 -5.94 4.57 4.45 3878.00 4875.10 10.94 -112.33 -5.42 -5.51 4.57 4.45 3889.00 4880.74 10.90 -111.69 -5.16 -5.74 4.57 4.45 3900.00 4881.17 11.33 -116.25 -5.30 -6.03 4.56 4.45 3914.00 4877.31 12.23 -125.12 -5.10 -7.13 4.56 4.45 3925.00 4884.76 11.96 -122.67 -5.57 -6.39 4.56 4.45 3942.00 4886.82 12.26 -125.61 -5.36 -6.90 4.56 4.44 3956.00 4887.58 12.65 -129.84 -5.70 -6.95 4.56 4.44 3969.00 4894.45 12.48 -127.98 -5.44 -7.04 4.56 4.44 3983.00 4895.53 12.85 -131.90 -5.75 -7.10 4.56 4.44 3997.00 4896.63 13.22 -135.79 -6.11 -7.10 4.55 4.44 4008.00 4899.34 13.45 -138.09 -5.96 -7.49 4.55 4.44 4020.00 4903.57 13.51 -138.86 -6.33 -7.19 4.55 4.44 4038.00 4905.64 13.80 -141.79 -6.37 -7.43 4.55 4.44 4051.00 4910.50 13.83 -141.93 -6.15 -7.68 4.55 4.44 4065.00 4908.37 14.51 -149.05 -6.61 -7.90 4.55 4.44 4079.00 4909.78 14.84 -152.65 -6.92 -7.92 4.54 4.44 4091.00 4913.95 14.92 -153.47 -6.85 -8.07 4.54 4.43 4102.00 4917.64 15.03 -154.79 -6.97 -8.07 4.54 4.43 4116.00 4918.00 15.49 -159.43 -7.05 -8.44 4.54 4.43 4133.00 4919.95 15.78 -162.48 -7.22 -8.57 4.54 4.43 4147.00 4920.63 16.21 -166.80 -7.34 -8.88 4.54 4.43 4161.00 4936.39 15.12 -156.04 -7.72 -7.39 4.54 4.44 4178.00 4932.84 15.97 -164.59 -7.54 -8.42 4.53 4.43 4190.00 4931.44 16.61 -170.99 -7.56 -9.05 4.53 4.43 4205.00 4931.75 17.05 -175.68 -8.01 -9.03 4.53 4.43 4215.00 4938.75 16.84 -173.68 -7.94 -8.90 4.53 4.43 4225.00 4932.96 17.90 -184.47 -8.24 -9.65 4.53 4.43 4241.00 4937.83 17.89 -184.60 -8.31 -9.58 4.53 4.43 4255.00 4950.00 17.17 -177.43 -8.36 -8.81 4.52 4.43 4264.00 4954.96 17.17 -177.47 -8.59 -8.58 4.52 4.43 4275.00 4950.61 18.08 -186.82 -8.89 -9.20 4.52 4.43 4289.00 4957.45 17.91 -184.98 -8.76 -9.15 4.52 4.43 4302.00 4955.75 18.56 -191.68 -8.95 -9.61 4.52 4.43 4316.00 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